Understanding scientific concepts such as atoms, molecules, cells, and biochemical processes can be challenging due to their abstract nature. Proteins, which provide structural and functional roles in skin, hair, muscles, hormones, enzymes, and various body parts, represent these challenges. Fundamental learning of the central dogma of protein synthesis (transcription and translation) is particularly difficult because proteins are small, invisible, and complex molecules. In addressing this issue, PolyTrans learning kit assists in transform the conceptual understanding of transcription and translation into a tangible, visual, and hands-on activity. The PolyTrans Kit is an affordable, attractive, mobile, user-friendly, and replicable educational tool. It includes colourful components of protein synthesis, secured by magnetic and strap fasteners. Additionally, an interactive Augmented Reality (AR) feature allows students to visualize and engage with an invisible process, enhancing their overall learning experience. This kit aims to provide an effective, enjoyable, and engaging experience for students, enhancing their interaction with the subject matter. By doing so, it fosters a deeper and more permanent understanding through dynamic participation. The effectiveness of the PolyTrans Kit was evaluated using a quasi-experimental design. Students were divided into two groups: one using conventional teaching methods and the other using the PolyTrans Kit. Levene's test confirmed the assumption of homogeneity of variance between the groups. The Independent Sample t-Test results indicated a statistically significant difference in biology test scores between the groups (t(33) = -3.18, p < 0.05). Specifically, the mean score of students using the PolyTrans Kit (mean = 84.5) was higher than that of students taught with conventional methods (mean = 76.8). In summary, the significant difference scores between the groups of students indicate PolyTrans shows an efficacy in fostering students understanding in protein synthesis learning