This study aims to examine job stress and job satisfaction among teachers in public secondary schools in Johor Bahru, Malaysia. Public secondary schools in Johor Bahru were selected as the research setting to explore the relationship between work stress and job satisfaction among teachers. Job satisfaction is used as an indicator of teachers' overall contentment with their work, and analyzing how work stress influences job satisfaction is crucial, as it directly affects the quality of knowledge transfer to students. Data was collected from 60 teachers using the convenience sampling method and analyzed using Statistical Package for Social Science (SPSS) version 23.0 and Partial Least Squares Structural Equation Modeling (PLS-SEM) with SmartPLS version 3. The results indicated that only one dimension, role ambiguity, had a significant negative relationship with job satisfaction, while the other three dimensions—workload, physical environment, and workplace conflict—did not show a significant impact. The study recommends that school management and government authorities implement strategies to address role ambiguity in order to enhance job satisfaction among teachers in public secondary schools.