Regular exposure to risks necessitates ongoing monitoring for laboratory personnel and students. Both parties must prioritise biosafety awareness. Insufficient empirical data suggests that students have inadequate knowledge, attitudes, and practices (KAP) when it comes to biosafety procedures. This raise worries regarding their safety practices in laboratories. Therefore, this study aims to address this issue particularly to assess biosafety knowledge, attitudes and practices among CFS IIUM students, across all biological students at the Centre for Foundation Studies (CFS) International Islamic University Malaysia (IIUM). A comprehensive cross-sectional investigation was carried out on the students. Data was gathered by means of an online self-administered questionnaire using Google Forms, targeting students enrolled in CFS IIUM. The results revealed there is a high level of attitude and practice towards biosafety protocols. Still, their knowledge needs slight improvement, particularly in specific areas like the number of containers used to transfer the biological sample. Training on biosafety is imperative to enhance knowledge, attitude and practice among CFS IIUM students. Furthermore, it is necessary to build efficient, operational, and well-executed biosafety policy procedures overseen by the institution. By addressing these deficiencies, safety standards can be improved, reducing the likelihood of accidents or exposure to dangers, and ultimately establishing safer laboratory settings for both students and staff.