Mud-Gambiered Silk, a distinctive traditional textile originating from the Lingnan region, features double-sided heterochromatic coloration along with excellent breathability and comfort. The dyeing process relies primarily on Dioscorea cirrhosa, whose tannins react with iron ions to generate the characteristic dark tones. However, limitations in the cultivation and availability of fresh tubers, combined with the laborious sequence of repeated dipping and drying, constrain the scalability and sustainability of this traditional method. Addressing these challenges requires diversifying dye sources and integrating complementary plant-based materials into the existing framework to ensure continued viability in contemporary production environments. This study investigates the comparative performance of Dioscorea cirrhosa and fermented persimmon lacquer in the Mud-Gambiered Silk dyeing process. Derived from unripe green persimmons through fermentation, persimmon lacquer operates under a similar principle: plant-derived tannins form black or dark brown compounds upon exposure to sunlight in the presence of iron. Through experimental comparisons, we analyzed variations in color depth and surface darkness after applying river mud, and evaluated the visual and practical outcomes. Findings indicate that persimmon lacquer achieves strong coloration effects with fewer dyeing cycles. Prolonged sun exposure enhances its chromatic outcome, producing a dark brown shade comparable to that achieved with Dioscorea cirrhosa, while reducing labor input and shortening the overall dyeing timeline. The research contributes a theoretical foundation for expanding the use of alternative dyeing materials in Mud-Gambiered Silk production. It also supports the modernization of heritage dyeing techniques by demonstrating the practical viability of persimmon lacquer, thereby opening new directions for sustainable innovation within contemporary textile applications. These findings highlight the value of integrating traditional knowledge with new natural dyeing materials to strengthen both environmental adaptability and artisanal continuity.