This study focusses on gender role representations in Spider-Man: Into the Spider-Verse. Gender roles in this film are categorized and analysed by employing Roland Barthes Semiotics. The application of semiotics method is appropriate in this study to conduct a thorough analysis on how gender roles are represented in the film. Denotative and connotative meanings are explored. Deliberation on the character physical representations is presented. Analysis towards the myths behind the gender roles representations is discussed. The analysis shows that no stereotypical characterizations of characters based on their physical representations and gender roles are represented in the film. The analysis shows that context is important in order to understand the meaning of gender role representations, whether it is stereotypical or not. In addition, the findings show that films each gender play multiple roles which are varied based on the context. Lastly, it shows that both men and women can have masculine and feminine traits in them as shown through the characters which display both masculinities and femininities despite their genders.