Efficacy of neem leaves extract and aloe vera as antimicrobial in hand sanitizer
List of Authors
  • Janani Murally , Norain Ahmad Nordin

Keyword
  • Hand sanitizer, Neem leaves extract, Aloe vera, S. aureus, E. coli

Abstract
  • Since the coronavirus disease pandemic of 2019 (COVID-19) is spreading quickly around the world, 99% alcohol-based hand sanitizer (ABHS) is utilized to minimize disease transmission. During the COVID-19 outbreak, isopropyl supply becomes limited. Herbal based hand sanitizers were used as an alternative way during pandemic to reduce the spreading of COVID-19. This study aimed to evaluate the efficacy of neem leaves extract and aloe vera as antimicrobials for Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) bacteria which can readily be found on the hands. Moreover, 30% isopropyl alcohol, citrus lemon oil, essential oil and vitamin E oil were also added in the formulations. Four different hand sanitizers were formulated which labelled as A1, A2, A3 and A4. The initial formulation, A1, is said to be the most effective because it contains the highest amount of neem leaf extract and aloe vera, and it is followed by A2, which contains less neem leaf extract and aloe vera. A3 does not contain neem leaf extract, while A4 does not contain aloe vera. All formulations contained the same amount of isopropyl alcohol, essential oil, vitamin E oil, and citrus lemon. Streaking methods on Tryptic Soy Agar plate and nutrient agar plate were used to evaluate the effectiveness of hand sanitizer against S. aureus and E. coli bacteria. The total colony forming unit (CFU) and percentage of effectiveness were calculated to compare the antimicrobial activity of all the formulations. The results revealed that a significant reduction in total bacterial colony counts of microbes after treatment with the formulations. Besides, pH value for A1, A2, A3 and A4 were also evaluated, and the results were 5.2, 5.3, 4.8, and 5.5 respectively. Among all four formulations, A1 showed the highest efficiency of microbial disinfectant for both S. aureus and E. coli bacteria by showing the reduction of microbes for 94.55% and 97.27% respectively since A1 contains the highest amount of neem leaves extract and aloe vera. It also has a skin-friendly pH value of 5.2 which is slightly acidic to control the bacterial infection and applicable since it is within the pH range of 4.5 to 6.5 found in human skin.

Reference
  • 1. Abuga, A. & Nyamweya, N. (2021). Alcohol-Based Hand Sanitizers in COVID-19 Prevention: A Multidimensional Perspective. Pharmacy, 9(1), 64.

    2. Ali, A. (1993). Textbook of Pharmacognosy. New Delhi, India: Publication and Information Directorate.

    3. Alzohairy, M. A. (2016). Therapeutics Role of Azadirachta indica (Neem) and their active constituents in diseases prevention and treatment. Evid Based Complement Alternat Med.

    4. Benalia, A., Derbal, K., Khalfaoui, A., Bouchareb, R., Panico, A., Gisonni, C., Crispino, G., Pirozzi, F., & Pizzi, A. (2021). Use of aloe vera as an organic coagulant for improving drinking water quality. Water (Switzerland), 13(15).

    5. Booq, R. Y., Alshehri, A. A., Almughem, F. A., Zaidan, N. M., Aburayan, W. S., Bakr, A. A., Kabli, S. H., Alshaya, H. A., Alsuabeyl, M. S., Alyamani, E. J., & Tawfik, E. A. (2021). Formulation and evaluation of alcohol-free hand sanitizer gels to prevent the spread of infections during pandemics. International Journal of Environmental Research and Public Health, 18(12).

    6. Chandanapalli, S. H., Tanish, B., Priya, D. P., Kumari, N. P., and Nayab, S. Hand sanitizers: is over usage harmful? World Journal of Current Medical and Pharmaceutical Research, 296-300.

    7. Chauhan, P., & Kumar, A. (2020). Development of a microbial coating for cellulosic surface using aloe vera and silane. In Carbohydrate Polymer Technologies and Applications, 1, 100015.

    8. Ghonmode W. N., Balsaraf O. D., Tambe V. H., Saujanya K. P., Patil A. K., and Kakde D. D. (2013). Comparison of the antibacterial efficiency of neem leaf extracts, grape seed extracts and 3% sodium hypochlorite against E. feacalis—an in vitro study. Journal of International Oral Health, 5(6), 61–66.

    9. Govindachari, T. R., Suresh, G., Gopalakrishnan, G., Banumathy, B., and Masilamani, S. (1998). Identification of antifungal compounds from the seed oil of Azadirachta indica . Phytoparasitica, 26(2),109–116.

    10. Hossain, M. A., Shah, M. D., and Sakari, M. (2011). Gas chromatography–mass spectrometry analysis of various organic extracts of Merremia borneensis from Sabah. Asian Pacific Journal of Tropical Medicine, 4(8), 637–641.

    11. Kokate, C., Purohit, A. P., and Gokhale, S. B. (2010). Pharmacognosy. Maharashtra, India: Nirali Prakashan.

    12. Krug, J. (2005). Kinetic pattern formation at solid surfaces. In Collective Dynamics of Nonlinear and Disordered Systems.

    13. Kulthanan, K., Maneeprasopchoke, P., Varothai, S., & Nuchkull, P. (2014). The pH of antiseptic cleansers. Asia Pacific Allergy, 4(1), 32.

    14. Mondali, N. K., Mojumdar, A., Chatterje, S. K., Banerjee, A., Datta, J. K., and Gupta, S. (2009). Antifungal activities and chemical characterization of Neem leaf extracts on the growth of some selected fungal species in vitro culture medium. Journal of Applied Sciences and Environmental Management, 13(1), 49–53.

    15. Natasha, F., Dutta, K., & Mollah, A. M. (2015). Antimicrobial and decontamination efficacy of neem, aloe vera and neem+ aloe vera in gutta percha (Gp) cones using escherichia coli and staphylococcus aureus as contaminants. Asian Journal of Microbiology, Biotechnology and Environmental Sciences, 17(4), 917–920.

    16. Odimegwu, J., & Ilomuanya, M. (2020). Citrutox ® Hand sanitizer-an innovative essential oil and alcohol-based sanitizer for preventing COVID19 infections.

    17. Plate Count Agar (Tandard Methods Agar). (2018). Retrieved from https://exodocientifica.com.br/_technical-data/M091.pdf

    18. Road, H., & Sheffield, S. (2000). A one year study of Escherichia coli O157 in raw beef and lamb products. 207–213.

    19. Sarmiento, W. C., Maramba, C. C., and Gonzales, M. L. M. (2011). An in vitro study on the antibacterial effect of neem (Azadirachta indica) leaf extracts on methicillin-sensitive and methicillin-resistant Staphylococcus aureus. PIDSP Journal, 12(1), 40–45.

    20. Shazia, M., Chandravanshi, J. S., Mansoor, S., Agarwal, A., Nandi, R. G., & Ganesh, N. (2021). Formulation of herbal hand sanitizer from Indian herbs. Lino, 11, 1-17.

    21. Selam, M. N. (2020). Hand sanitizers marketed in the streets of addis ababa, ethiopia, in the era of covid-19: A quality concern. Risk Management and Healthcare Policy, 13, 2483-2487.

    22. Surjushe, A., Vasani, R., and Saple D. G. (2008). Aloe vera: a short review. Indian J Dermatol, 53(4),163-6.

    23. Tryptic Soy Agar. (2014). Retrieved from http://www.dalynn.com/dyn/ck_assets/files/tech/PB81.pdf

    24. Wilson, J. B. (1962). Egg yolk factor of Staphylococcus Aureus I. nature of the substrate and enzyme involved in the egg yolk. 516–521.