Sedentary work is a significant occupational hazard in the healthcare sector, contributing to a high prevalence of work-related musculoskeletal disorders (MSDs). In Malaysia, healthcare professionals face increased risks due to prolonged periods of sitting associated with administrative tasks. This study aimed to investigate the effectiveness of sit-stand workstations as an ergonomic intervention to mitigate sedentary behaviour and reduce musculoskeletal discomfort among employees at the Sarawak Heart Centre. A quantitative pre-post intervention design was employed with 30 desk-based healthcare employees. Participants used sit-stand workstations for four weeks. Data on sitting duration, movement frequency, and musculoskeletal discomfort were collected before and after the intervention using a structured questionnaire, including the Cornell Musculoskeletal Discomfort Questionnaire (CMDQ). The intervention yielded significant positive outcomes. The number of employees sitting for more than four hours daily was halved. Participants reported an increased standing frequency and a significant improvement in perceived mobility at work. Musculoskeletal discomfort decreased substantially; the number of participants reporting moderate to severe discomfort dropped from 24 to 8, with severe discomfort being eliminated. The most significant reductions in discomfort were observed in the lower back (from 61% to 42%), shoulders (from 38% to 23%), and neck (from 37% to 20%). The implementation of sit-stand workstations proved to be a highly effective strategy for reducing sedentary behaviour and alleviating musculoskeletal strain among healthcare professionals. The findings provide strong evidence supporting the adoption of such ergonomic solutions to create healthier work environments in healthcare settings.