Prevalence of Escherichia Coli from raw fresh milk produced from farm in Negeri Sembilan
List of Authors
  • Aimi Nadia Ramli , Farra Amira Mohamed

Keyword
  • Raw milk, E.coli, milk contamination, biochemical test, antibiotic susceptibility, MAR Index

Abstract
  • Milk is an extremely nutritious source of food as it contains colloidal suspension of protein, fat, carbohydrate, vitamin and mineral. Drinking raw or undercook milk may harbor the most common foodborne pathogenic bacteria if prepared inexpertly. The aim of this study was to detect the presence and susceptibility level of Escherichia coli towards selected antibiotics in cow raw fresh milk produced on dairy farms in Negeri Sembilan. The cow raw fresh milk samples were randomly selected from Pusat Perkhidmatan Industri Tenusu (PPIT) Kuala Pilah, Negeri Sembilan. Escherichia coli were isolated on Eosin Methylene Blue agar (EMBA) and confirmation was performed by using a series of biochemical test. The resuts revealed that only six out of nine isolates were positive for Escherichia coli, which exhibits a high resistance (100%) towards Vancomycin (VA5). In fact, none of the Escherichia coli isolates were resistant towards Gentamicin (CN10) and Streptomycin (S10). In addition, the multiple antibiotics resistance (MAR) indices were arranged from 0.2 to 0.4. The presence of Escherichia coli in the samples understudy had revealed that there was a high probability of contamination occurred due to the inadequate hygiene condition performed by the farmers while milking, storing and transporting the milk.

Reference
  • 1. Theresa, A., Nicklas, Dr PH, LN. (2003). “Calcium Intake Trends and Health Consequences from Childhood through Adulthood”. J Am Coll Nutr, 22(3): 40-56.

    2. Syed, Z. H., Shaker, M., Gulve, R. M., and Iqbal, M. A. (2014). “Bacterial Analysis of Raw and Packed Milk of Beed City”. Journal of advances in Applied Sciences and Technology, 1(1): 53-58 pp.

    3. Vahedi, M., Nasrolahei, M., Sharif, M., and Mirabi, A. M. (2013). “Bacteriological study of raw and unexpired pasteurized cow’s milk collected at the dairy farms and super markets in Sari city in 2011.” J Prev Med Hyg, 54: 120-123 pp.

    4. Mohamed, M. A. A., and Gihan, K. A.-L. (2014). “Public Health Risk of Some Milk Borne Pathogens.”

    5. Beni-Suef Univiersity Journal of Basic and Applied Sciences, 3: 209-215 pp.

    6. Lye, Y. L., Afsah-Hejri, L., Chang, W. S., Loo, Y. Y., Puspanadan, S., Kuan, C. H.,

    7. Goh, S. G., Sharil, N., Rukayadi, Y., Khatib, A., John, Y.H.T., Nishibuchi, Y. and Son, R. (2013). “Risk of Escherichia coli O157:H7 transmission linked to the consumption of raw milk.” International Food Research Journal, 20(2): 1001-1005 pp.

    8. Bakr, W. M., Hazzah, W. A., and Abaza, A. F. (2011). “Detection of Salmonella and Vibrio Species in Some Seafood in Alexandria.” Journal of American Science, 663-669 pp.

    9. Samuel, L., Marian, M. M., Apun, K., Lesley, M. B., & Son, R. (2011). “Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis.” International Food Research Journal, 971-976.

    10. Krumperman, P. H. (1983). “Multiple Antibiotic Resistance Indexing of Escherichia coli to identify High-Risk Sources of Fecal Contamination of Foods”. Applied and Environmental Microbiology, 46, 165-170 pp.

    11. Lim, J. Y., Yoon J. W., Hovde C. J. (2013). “A Brief Overview of Escherichia coli O157:H7 and Its Plasmid O157.” J Microbiol Biotechnol, 20(1): 5-14 pp.

    12. Guodong, W., Tong, Z., and Michael, P. D. (1996). “Survival and Growth of Escherichia coli O157:H7 in Unpasteurized and Pasteurized Milk”. Journal of Food Protection, 60 (6): 610-613 pp.

    13. Melisa, A., Patrice, H., Stacyann, H., Princena, M., Donovan, M., Ruby, A., and Lindo. (2011). “The Microbial Content of Unexpired Pasteurized Milk from Selected Supermarkets in a Developing Country.” Asian Pasific Journal of Tropical Biomedicine, 1(3): 205-211 pp.

    14. Hemraj, V., Diksha, S., & Avneet, G. (2013). “A Review on Commonly Used Biochemical Test for Bacteria.” Innovare Journal of Life Science, 1-7.

    15. National Committee for Clinical Laboratory Standards (NCCLS). (2004). Performance standards for Antimicrobial Susceptibility Testing, Fourteenth International Supplement Document Number NCCLS/CL.SI M100-S14 Clinical and Laboratory Standard Institute. Villanova, P. A., USA.

    16. Alharbi, N. S., Khaled, J. M., Kadaikunnan, S., Alobaidi, A. S., Sharafaddin, A. H., Alyahya, S. A., Almanaa, J. M., Alsughayier, M. A., and Shehu, M. R. (2018). “Prevalence of Escherichia coli Strains Resistance to Antibiotics in Wound Infections and Raw Milk”. Saudi Journal of Biological Sciences.

    17. Pokhrel, H., Baishya, S., Phukan, B., Pillai, D., & Rather, M. A. (2018). Occurrence and Distribution of Multiple Antibiotic Resistance Bacteria of Public Health Significance in Backwaters and Aquaculture Farm. International Journal of Current Microbiology and Applied Science, 7, 975-987 pp.

    18. Thaker, H. C., Brahmbhatt, M. N., and Nayak, J. B. (2012). “Study on occurrence and antibiogram pattern of Escherichia coli from raw milk samples in Anand, Gujarat, India”. Veterinary World, 5(9): 556-559 pp.

    19. Sieuwerts, S., de Bok, F. A., Mols, E., de Vos, W. M., & van Hylckama Vlieg, J. E. (2008). “A simple and fast method for determining colony forming units.” Letters in Applied Microbioogy, 275-278.

    20. Adzitey Frederick. (2011). “Escherichia Coli, it Prevalence and Antibiotic Resistant in Malaysia: A Mini Review”. Microbiology Journal, 1: 47-53.

    21. Saleha, A. A. (2002). “Isolation and characterization of Campylobacter jejuni from boiler chickens in Malaysia”. Int. J. Poult. Sci, 1: 94-97 pp.

    22. Arumugaswarny, R. K., Rusul, G., Hamid, S. A., Cheah, C. T. (1995). “Prevalence of Salmonella in raw and cooked foods in Malaysia”. Food Microbiol, 12: 3-8 pp.