The acceptance and readiness towards inclusive education has been a complex issue over the centuries around the globe and educators have different views or attitudes on the implementation of inclusive education in the mainstream classrooms. Hence, this research aimed to investigate the pre-service teachers’ attitudes toward inclusive education among pre-service teachers of the Faculty of Education, UiTM Puncak Alam. The data were obtained by online survey by distributing to 112 pre-service teachers from various options including Teaching English as a Second Language (TESL), Mathematics, Physics, Biology, Chemistry, Visual Arts Education and Physical and Health Education who have undergone their teaching practicum for 14 weeks in secondary schools. The instrument was taken from past research by Walker (2012) on the attitudes toward inclusive education among teachers. The findings suggested that the pre-service teachers have positive attitudes toward inclusive education. Also, pre-service teachers showed a negative level of readiness to teach an inclusive classroom. However, there is a significant difference in pre-service teachers’ attitudes toward inclusive education among majors in which Physical and Health Education pre-service teachers showed more positive attitudes toward inclusive education compared to other pre-service teachers.